Appendix 4 of Mansergh, Mol Vis 2015; 21:61-87.

Appendix 4:

q-PCR results of all 42 genes tested, including original array results for comparison. Data are shown in both table and graph format. Column K lists which changes were noted as statistically significant via array, Column M notes whether these were confirmed or not (yes/no). t test results for Q-PCR are given in columns O and P. Values of p<0.05 were considered significant for q-PCR. The direction of expression changes were confirmed by Q-PCR for 90.5% of cases for FACS comparison of Rho-eGFP+ve versus negative cells, and for 94% of changes noted between pairs of unamplified samples. Significant changes via q-PCR t test were 88% and 91% of the same data sets. x-axis: samples; RPC=RPCs, Pn1=pn1 retina, FACS-=Rho-eGFP negative cells, FACS+=Rho-eGFP positive cells, Pn5=pn5 retina, Ad ret=adult retina. y-axis: normalized fold changes where pn1 is set at 1. To access these data, click or select the words "Appendix 4".