Appendix 1 of Mohd Khalid, Mol Vis 2015; 21:1185-1190.
T7Prom and T7Term represent both T7Promoter and T7Terminator universal sequencing primers that were tagged to all exon-specific PCR primer sequences. T7Prom was tagged to Forward primers while T7Term was tagged to Reverse primers. For each primer, sequences in uppercase belonged to T7Prom/Term and sequences in lowercase were exon-specific. a %GC primer was calculated using the GC Calculator program. b Predicted amplicon size (bp) was calculated using the UCSC In-Silico PCR program available at https://genome.ucsc.edu/cgi-bin/hgPcr. To account for T7 sequences that were present on both Forward and Reverse primers, additional 39bp was added to the amplicon size predicted by the aforementioned program. To access the data, click or select the words “Appendix 1.”