Figure 7. Isolation of CD34⁺/VEGFR2⁺ mononuclear cells. Single positive controls and an unstained control were used to set the appropriate compensation values necessary to assign the double positive gating strategy (A, B, and C). Whole cells were separated from debris and doublets using the area and width pulses for forward and side scatter (D). The box identifies all double-positive cells that exhibit sufficient fluorescence of both markers to be identified—these were the cells that were sorted and collected.