Inhibition of both HIF1α and HIF-2α diminishes Bcl-2 levels without affecting BAX levels. Cell lysates collected from cells
treated with 0.5 µM, 5 µM, and 50 µM of HIF-1α/HIF-2α translation inhibitors were analyzed with western blot analysis for
BAX and Bcl-2 levels. There was no change in the protein levels of BAX, and a significant decrease in the levels of Bcl-2
coupled with the loss of VEGF (Figure 4L
) was observed with the double translation inhibitor (A
represent the corresponding densitometry analysis.