Figure 4 of Wang, Mol Vis 2012; 18:3049-3056.


Figure 4. BSA fraction V significantly improved polymerase chain reaction (PCR) yield in iris DNA. A set of parallel PCR was performed to compare the effect of BSA on PCR amplification of DNA obtained from iris tissues. The PCR conditions used in both groups were the same as that described in the method section and Figure 2, with the exception that the BSA+ group contained 1 μg of BSA per 1 μl of PCR solution. Each lane represented an individual iris DNA sample, with the number an arbitrary label assigned to each donor. The “i” following each number indicates that the DNA is of iris origin. Note: M=Marker (1 kb DNA ladder, Invitrogen); NTC=non-template control (negative control).