Figure 3. rhCTGF induced proliferation in primary tenon fibroblasts. HTFs were starved for 24 h and (A) subsequently stimulated for 48 h with increasing CTGF concentrations up to 4 μg/ml and 10% FCS as positive control and (B) for different time points between 24 and 72 h with a concentration of 4 μg/ml rhCTGF. In both experiments [3H]-thymidine DNA incorporation was measured after a 24 h incubation intervall by RITA.