Molecular Vision: Abu-Amero, Mol Vis 2011; 17:667-672. Table 1

Table 1. Primer sequences, PCR annealing temperature, and amplicon size for VSX1 PCR amplification.

Exon	Primer sequence	Annealing temperature (°C)
VSX1–1F	TGTAAAACGACGGCCAGTCAGCTGATTGGAGCCCTTC	60
VSX1–1R	CAGGAAACAGCTATGACCGGGGCGATGGTCTGTGAC
VSX1–2F	TGTAAAACGACGGCCAGTCCCAAGAGGTTCATAACTTCAA	60
VSX1–2R	CAGGAAACAGCTATGACCTATCATGCCGGGCCATAAAT
VSX1–3aF	TGTAAAACGACGGCCAGTCTGGACAGCAGAGGAAGCA	60
VSX1–3aR	CAGGAAACAGCTATGACCTTGGCTATAGAGAAGGGACTGC
VSX1–3bF	TGTAAAACGACGGCCAGTTGGACAGCAGAGGAAGCAG	60
VSX1–3bR	CAGGAAACAGCTATGACCTCAGGGGCAGATAATATACTCCA
VSX1–4F	TGTAAAACGACGGCCAGTGCCCCTCTTCACTGTCTCTTC	60
VSX1–4R	CAGGAAACAGCTATGACCTTGCTTTGCTTTGGAAATGG
VSX1–5aF	TGTAAAACGACGGCCAGTTGGGATTTAGAGAACAATAAGAAGG	58
VSX1–5aR	CAGGAAACAGCTATGACCCAGTGAAATCATTTTTGAACTTCG
VSX1–5bF	TGTAAAACGACGGCCAGTCAGATGAGTGACCTAGGGAAAA	57
VSX1–5bR	CAGGAAACAGCTATGACCTGTCACATGTCCCCAGATTG