Figure 1. Human corneal endothelial cell examination. Inverted phase-contrast imaging (A) and immunofluorescent ZO-1 staining (B) of the human corneal endothelial cells. Human corneal endothelial cells were observed in mosaic pattern. Immunofluorescence with anti-ZO-1 antibody, a general marker of the tight junction, revealed the morphology. The nuclei were stained using Hoechst 33342 (blue). Magnification, 200×.