Figure 3. Non-optic cup lip derivatives populate the posterior neural retina. A-E: The optic cup was targeted with retrovirus, as described in Figure 1E, and reincubated until E5 (A). B: Whole mount view of GFP expressing cells (arrow), located at a distance from the OCL. C, D: Transverse sections through the GFP expressing region. GFP+ cells do not populate the ciliary body (Collagen IX signal) or the margin between the ciliary body and neural retina (red arrow). Asterisks indicate OCL. E, F: GFP+ cells are found only in the Hu+ neural retina. Boxed region in E is represented at higher magnification in F. G-O: Results of optic cup labeling, examined at E10. G, H: GFP+ cells are not immediately detectable in whole mount view of head. I, J: Infected eye dissected from head, nasal (N) and temporal (T) orientation as indicated. No GFP signal is seen in anterior view of eye. K, L: GFP+ cells are seen at the posterior-dorsal aspect of the optic cup (bracketed by green arrowheads). Curved line indicates a tear in the RPE. L: Slightly higher magnification of K. M-O: Sections through eye in K. GFP+ cells are only found in the posterior retinal domain (green arrowheads). The ora serrate/CMZ, defined as the junction between the ciliary body (Collagen IX signal, M) and neural retina (Visinin signal, N), is indicated by the red arrow. GFP+ cells are not found at the CMZ. Asterisk indicates OCL. Note, isolated eye tissue is delicate and does not adhere well upon sectioning. In panel N the tissue has flipped during processing (white line). Boxed are in N shown at higher magnification in O. Scale Bars: 100 µm. N; Nasal, T; temporal, L; Lens.