Figure 3. Arginine vasopressin
receptors in the retina detected by whole-retina reverse
transcriptase–polymerase chain reaction.
A: Arginine
vasopressin (AVP) receptors were detected from a whole wild-type
rat retina by reverse transcriptase (RT)–PCR with the indicated
primer pairs (
Table 1). V1a and V1b
receptors were detected. A V2/
ARHGAP4 band was also
detected, but by cloning and sequencing the RT–PCR product, we
concluded that the V2/
ARHGAP4 was from the
ARHGAP4
gene, which overlaps the V2 receptor gene and is expressed from
the opposite DNA strand of the V2 receptor gene.
B: No
AVP receptor was detected by RT–PCR in dissociated
AVP-eGFP-positive cells, as shown in
Figure 2B.