vasopressin mRNA was detected by reverse
transcriptase–polymerase chain reaction from dissociated
GFP-positive cells and the whole retina. A
of the arginine vasopressin (AVP)-eGFP transgenic rat retina.
Under a fluorescence microscope, we identified AVP-eGFP-positive
cells by GFP fluorescence. Scale bars are 20 μm. B
Reverse transcriptase (RT)–PCR from a set of 20 dissociated
GFP-positive cells. A faint endogenous AVP band (white arrow)
and an AVP-eGFP band were detected by the indicated primer pairs
) after 45 cycles of RT–PCR. C
from the wild-type whole retina. An endogenous AVP
was also detected.