Figure 2. Endogenous arginine vasopressin mRNA was detected by reverse transcriptase–polymerase chain reaction from dissociated GFP-positive cells and the whole retina. A: Dissociation of the arginine vasopressin (AVP)-eGFP transgenic rat retina. Under a fluorescence microscope, we identified AVP-eGFP-positive cells by GFP fluorescence. Scale bars are 20 μm. B: Reverse transcriptase (RT)–PCR from a set of 20 dissociated GFP-positive cells. A faint endogenous AVP band (white arrow) and an AVP-eGFP band were detected by the indicated primer pairs (Table 1) after 45 cycles of RT–PCR. C: RT–PCR from the wild-type whole retina. An endogenous AVP band was also detected.