Figure 2. Endogenous arginine
vasopressin mRNA was detected by reverse
transcriptase–polymerase chain reaction from dissociated
GFP-positive cells and the whole retina.
A: Dissociation
of the arginine vasopressin (AVP)-eGFP transgenic rat retina.
Under a fluorescence microscope, we identified AVP-eGFP-positive
cells by GFP fluorescence. Scale bars are 20 μm.
B:
Reverse transcriptase (RT)–PCR from a set of 20 dissociated
GFP-positive cells. A faint endogenous AVP band (white arrow)
and an AVP-eGFP band were detected by the indicated primer pairs
(
Table
1) after 45 cycles of RT–PCR.
C: RT–PCR
from the wild-type whole retina. An endogenous
AVP band
was also detected.