Figure 4. HPLC-GPC fractionation of the soluble proteins in concentric layers (numbered from the outside in) isolated from (A) a 130-day-old PY lens and (B), a 4-year-old eastern gray kangaroo lens. C: SDS–PAGE patterns of the three major peaks from the 4-year-old lens layers. D: Proportions of the soluble α- (•••), β- (•••), γ- (•••), and μ- (•••) crystallins from the 4-year-old lens layers (calculated from Figure 4B) as a function of the accumulated dry weight from the center.