Molecular Vision: Surgucheva, Mol Vis 2011; 17:2878-2888. Table 1

Table 1. Primers used for the amplification and in vitro mutagenesis.

1	F 5′-TATAGGTACCAATTTATGGCAGTCATTA-3′	Amplification
2	R 5′-ATATGAGCTCGCTGGCCCGTGGCTGGAT-3′
3	S 5′-GTCCTTTTCTAGTGTATTGTGTTTGTTAATATTTG-3′	A→G
4	Compl 5′-CAAATATTAACAAACACAATACACTAGAAAAGGAC-3′
5	S 5′-GCTCCCACTAATGTGGTATTTAACTTTTGAAAGATCTTTAG-3′	NFκB (−1537)
6	Compl 5′-CTAAAGATCTTTCAAAAGTTAAATACCACATTAGTGGGAGC-3′
7	S 5′-GCTTTAAATAATTCTACAATATTTTGTGTATTTTGC-3′	HNF-3
8	Compl 5′-GCAAAATACACAAAATATTGTAGAATTATTTAAAGC-3′
9	S 5′-CAGAAAATATCGGTAATAAAAAAAGTTAAAGATCTTC-3′	Pou-IV
10	Compl 5′-GAAGATCTTTAACTTTTTTTATTACCGATATTTTCTG-3′
11	S 5′-GGAGGCTCCCTCCCCCCCCTCCAGCGC-3′	Sp1 (−236)
12	Compl 5′-GCGCTGGAGGGGAGGGAGGGAGCCTCC-3′
13	S 5′-GACCCGGCGCAGCACACCAACCGCGAGCAGAAC-3′	Ets
14	Compl - 5′-GTTCTGCTCGCGGTTGGTGTGCTGCGCCGGGTC-3′
15	S 5′-CGCTGCGGGCGCTTGCTGCCAGAACCTTG-3′	Sp1 (−124)
16	Compl 5′-CAAGGTTCTGGCAGCAAGCGCCCGCAGCG-3′
17	S 5′-CTTAAAGCACAGCCCATCACAGTTTTCATCCAGC-3′	NFκB (−40)
18	Compl 5′-GCTGGATGAAAACTGTGATGGGCTGTGCTTTAA-3′

1 and 2 - human CAV-1 promoter-specific primers used to amplify 2, 465 bp PCR product: forward primer containing Kpn1 site and reversed primer containing SacI site. Three and 4 - primers used for the replacement of A on G in position −2,388 upstream from the start codon in CAV-1 promoter region (Figure 1B, modification 1). Primers 5 – 18 used for the deletion of putative TF binding sites. Other details are presented in Methods and Table 2.