Figure 2. Typical nuclei and nuclear fragment cortex inclusions in a 25-month-old C57BL/6 mouse. A comparison of nuclei and nuclear cortical fragments in the bow areas of a typical 25 month-old C57BL/6 mouse eye section using both Dapi (A) and H&E (B) stains. All of the included nuclei stained with Dapi (white) were also stained blue by Hematoxylin. All other H&E and Dapi images demonstrated the same correspondence (not shown), but Dapi was much easier to score at lower magnification so was generally used in scoring and grading retained nuclei and nuclear fragments. Original magnification was 200×. Other details are as described in legend to Figure 1.