Molecular Vision: Kubista, Mol Vis 2011; 17:2080-2092. Table 2

Table 2. Target sequences of the oligonucleotides used in the MLPA assay.

Target Gene	Oligonucleotide side	MLPA Oligonucleotides (5′ - 3′) hybridization sequences*	Product length (bp)
CFH	LO	gcctccttgttttaacagtctggct	100
(Intron 1)	RO	P-cagggggtcactgcaggctactaagaattgggt
CFHR3	LO	CAAAAGCGCAGACCACAGTTACATGTAC	104
(Exon 3)	RO	P-GGAGAAAGGCTGGTCTCCTACTCCCAGATGCATC
CFH	LO	GTCAGACAGTTATCAGTATGGAGAAGAAGTTAC	108
(Exon 18)	RO	P-GTACAAATGTTTTGAAGGTTTTGGAATTGATGG
CFHR1	LO	CAACATTTCATGTGTAGAACGGGGCTGGTCCACC	112
(Exon 3)	RO	P-CCTCCCAAATGCAGGTCCACTGgtaagtacaatgct
CFHR4	LO	CAGAAATTCAACATGGAGGTCTATATTATAAGAGTT	116
(Exon 2)	RO	P-TGCGTAGACTATACTTTCCAGCAGCTGCAGGACAATCT
CFHR2	LO	cagggattagtacagtgcaatctctgggggctgttattcc	120
(Intron 3B)	RO	P-gtttacaacaccatgcaataaccttatatgaataaagc
CFHR5	LO	CAACACACCAACTTAAGAGGTGCAAAATAGCAG	124
(Exon 7)	RO	P-GAGTTAATATAAAAACATTACTCAAGCTATCTGGGAAAGAATTTAATCA
PDCD8	LO	CGCAAATACAACAGGTATCAGAACTGCTGGCCCCAGATTAAG	128
(Exon 8)	RO	P-CTTCAGATGGTGAACTCTGTGCACTTCCACCCATGCAGTCACCT
SS18	LO	GGCAATCATATGATGGGTCAGAGACAGATTCCTCCCTATAGAC	132
(Exon 6)	RO	P-CTCCTCAACAGGgtaagattccatttggaaaatttgctacttaatgt

*Sequences within an exon are shown in upper case, while sequences in introns are presented in lower case. The LO begins with the forward primer sequence 5′-TGG GTC CCT AAG GGT TGG A-3′ followed by the sequence shown in the table. The 5′-end of the RO is phosphorylated (P) and the sequence shown in the table is followed by the complement of the reverse primer 5′-TCT AGA TTG GAT CTT GCT GGC AC-3′. Figure 1 and the methods provide additional details.