Figure 5. Canolol induced mRNA expression levels of catalase, HO-1, GST-pi, and Nrf-2 in ARPE-19 cells. A: The ARPE-19 cells were incubated with different concentrations of canolol for 24 h and were then collected to detect the mRNA expression levels of catalase, HO-1, GST-pi, and Nrf-2 by RT–PCR assay. B: Quantitative analyses of the relative density of mRNA levels in ARPE-19 cells (n=3). GAPDH was used as an internal control for sample normalization. The results were represented by a mean±SEM (n=3). Data was expressed as a percentage of the untreated control. The single asterisk indicates p<0.05 and the double asterisk indicates p<0.01 versus the control.