Figure 3. Canolol reduced t-butyl hydroxide (t-BH)-induced intracellular reactive oxygen species (ROS) generation in ARPE-19 cells. A: The ARPE-19 cells were incubated with 100 μM and 200 μM of canolol or 1 mM of N-acetyl cystein (NAC) for 24 h, and were then treated with 150 μM t-BH for 4 h. The intracellular ROS was examined by a fluorescence microscope (A) and a fluorescence spectrophotometer (B) using H₂DCFDA. The results were represented by a mean±SEM (n=3). Data was expressed as a percentage of the untreated control. The double asterisk indicates p<0.01 versus t-BH-induced cells without canolol pretreatment. The white columns in B represent treatment with different concentrations of canolol and in the absence of t-BH.