Figure 2. Canolol protected against t-butyl hydroxide (t-BH)-induced cell death in ARPE-19 cells. A: The ARPE-19 cells were incubated with 0.5% ethanol or different concentrations of canolol (25–200 μM) for 48 h. B: The cells were treated with t-BH at doses of 100, 120, 150, and 200 μM for 24 h. C: The cells were pretreated with canolol (50–200 μM) or N-acetyl cystein (NAC; 1 mM) for 24 h, then exposed to t-BH (150 μM) for 24 h. The cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide (MTT) assay. The results were represented by mean±SEM (n=4) from three independent experiments. Data was expressed as a percentage of the untreated control. The double asterisk indicates p<0.01 versus the control vehicle (A), untreated control (B), or t-BH-induced cells without canolol pretreatment (C). D: Morphology of ARPE-19 cells exposed to t-BH (150 μM) for 24 h with/without canolol pretreatment.