Figure 2. Quantitative PCR for microarray validation. Array validation was performed by quantitative RT–PCR for 5 genes selected from the list in Table 1. Rhodopsin was used as a retina-relevant reference gene. qPCR is found in excellent agreement with the outcome of microarray analysis. The results of qPCR were averaged from three independent experiments, with the error bar indicating the standard error of the mean. Dashed lines at fold differences of 1 and −1 indicate no difference of gene expression between the inferior and superior regions.