Table 3 of Asomugha, Mol Vis 2010; 16:476-494. Table 3 of Asomugha, Mol Vis 2010; 16:476-494.
Table 3. DeCyder software analysis highlighted statistically significant spots, based on technical replicates, differentially expressed in nuclear and cortical regions (p<0.05) from the 2D-DIGE gel of a 69 year-old lens (Figure 3) and crystallin species were then identified by ESI-QTRAP LC-MS/MS (Appendix 1).
| Spot number | Crystallin species | Mr (kDa) | Nucleus/cortex |
|---|---|---|---|
| 6 | αA chain, αB, βA3, γD, γS | <20 | 1.24/1 |
| 8B | αB, βA3 | <20 | 2.08/1 |
| 11 | αA, βA3, βA4 | <20 | 3.14/1 |
| 11B | αA, αB chain, βA4 | <20 | −1.61/1 |
| 12 | αA, γS | 20 - 35 | −1.97/1 |
| 12B | αA, αB chain, βA3, βB1, βB2, γC, γS | 20 - 35 | −1.51/1 |
| 14B | βB2 | 20 - 35 | −2.06/1 |
| 15B | βA3, βA4, βB2, γS | 20 - 35 | −2.21/1 |
| 17 | Truncated βB1, βB2 | 20 - 35 | 2.01/1 |
| 23 | NI | >35 | 3.00/1 |
| 24 | NI | >35 | 2.17/1 |
| 25 | NI | >35 | 2.84/1 |
| 26 | NI | >35 | −3.14/1 |
| 27 | NI | >35 | 1.96/1 |
| 29 | NI | >35 | 2.49/1 |
| 30 | NI | >35 | 3.09/1 |
| 31 | NI | >35 | 2.18/1 |
| 32 | NI | >35 | 3.42/1 |
| 33 | NI | >35 | 3.58/1 |
| 34 | NI | >35 | 3.65/1 |
| 35 | αA, filensin | >35 | 3.91/1 |
| 36 | NI | >35 | 3.40/1 |