Figure 5. Confocal microscopy of laser-induced choroidal neovascularization (CNV) shows the extent of endothelial cell proliferation in neuroprotecin D1 (NPD1)-treated animals. A, B: In this choroidal flatmount preparation from an NPD1-treated animal at 7 days post treatment, the retina has been removed to expose a typical laser lesion through Bruch’s membrane and into the choriocapillaris. A: Maximum projection images of immunolocalization for cytoskeleton (f-actin, green), nuclei (Heochst, blue), and endothelial cells (isolectin B4, red) are shown on the left, along with a merged image of the three labels, as in Figure 4. B: The same lesion as in A, presented as an orthogonal cut view. The large square image represents a single layer in a confocal stack of images, as viewed above from the retina, with the three labels merged. Lateral views of this image stack are shown at the top and to the right; the blue line in these views indicates the level within the stack of the view shown in the square. The lateral view at the top is from the position of the green line in the large square; the lateral view to the right is from the position of the red line in the large square. C, D: Images, organized the same as in A and B, through a typical retinal laser lesion of an NPD1-treated animal at 14 days post treatment. This lesion is presented at the right as an orthogonal cut view. White circles represent the 50-µm diameter initial laser lesion. Magnification bars are 50 µm.