Figure 5. Expression of lens crystallins in corneal limbal rim of adult human donor or cultured corneal epithelial cells. The limbal rims were obtained during preparation of corneal grafts for keratoplasty. A: Routine immunohistochemistry protocol was performed using proper combination of proper anti-crystallin primary and HRP-conjugated secondary antibodies. The sections were developed with 3, 3′-diaminobenzidine and counterstained with hematoxylin. B: Part of the limbal rims was used for culture of corneal epithelial cells and stained as described in the Figure 4 legend.