Figure 5 of Sheline, Mol Vis 2010; 16:2639-2652.

Figure 5. Plastic sections confirm protection of ONL by pyruvate and nicotinamide against LD. A: Plastic sections were cut from eyes along a superior to inferior meridian encompassing the optic nerve and stained with 0.1% Toluidine Blue. Representative photo-micrographs were taken of the mid superior and mid inferior regions from each of the groups as designated in Figure 4. Bar represents 50 microns. B: The mean number of nuclei at increasing distance from the optic nerve on the superior and inferior sides was averaged and plotted as a function of distance from the optic nerve for each of the experimental conditions above (n=6). # signifies difference from LD at p<0.05 by one-way ANOVA and a Bonferroni test.