Figure 5. Plastic sections confirm
protection of ONL by pyruvate and nicotinamide against LD.
A:
Plastic sections were cut from eyes along a superior to inferior
meridian encompassing the optic nerve and stained with 0.1% Toluidine
Blue. Representative photo-micrographs were taken of the mid superior
and mid inferior regions from each of the groups as designated in
Figure 4.
Bar represents 50 microns.
B: The mean number of nuclei at
increasing distance from the optic nerve on the superior and inferior
sides was averaged and plotted as a function of distance from the optic
nerve for each of the experimental conditions above (n=6). # signifies
difference from LD at p<0.05 by one-way ANOVA and a Bonferroni test.