Figure 1. Expression analysis of Cerkl in the mouse eye. A: Reverse Transcription-Polymerase Chain Reaction (RT–PCR) analysis of Cerk (412 bp) and Cerkl expression in mouse brain (Br) and retina (Ret). Cerkl expression was tested using primers located in exons 1 and 2 (see Figure 2B). The two PCR products obtained for Cerkl (503 and 363 bp) represent different splice-isoforms. B: RT–PCR analysis of Cerkl in the mouse eye at different developmental time points: embryonic day 14 (E14), newborn (P0), and 5 months (5 mo). The analysis indicates Cerkl expression at all time points tested. β-actin (Actb; 437 bp product) served as an internal control for RNA quality and quantity. M: size marker.