Figure 5. Computer-assisted identification and quantification of nuclei in a 60° sector (AS) of the anterior lens epithelium. A: Nuclei are identified using the count nuclei application. Colors indicate nuclear size (large nuclei are shown in warmer colors). B: Following image segmentation, nuclei lying within sector AS are counted (green). The arc of the sector is located at d, the fiduciary nucleus (see Figure 3 and Figure 4). C: A higher magnification view of the boxed area in B. The position of the centroid (center of mass) of each nucleus is calculated (indicated by a white dot). Only nuclei with centroids located within AS are included in the analysis.