Figure 3 of Bassnett, Mol Vis 2010; 16:2294-2300.


Figure 3. Orthographic projections of anterior or equatorial regions of the mouse lens. A: Three-dimensional rendering of an anterior lens quadrant, showing EdU-positive nuclei (yellow) and total draq5-stained nuclei (red). An animated version of this panel can be viewed at animation1. EdU-positive cells are relatively numerous near the lens equator but rare or absent in the anterior polar (AP) region. B: Two-dimensional orthographic projection of the data shown in A. The EdU-positive cells form recognizable “constellations.” One such constellation is formed by four nuclei: a, b, c, d. C: Two-dimensional orthographic projection of the lens equator. The constellation a, b, c, d, is also discernible in this orientation. D: High magnification view of the boxed region shown in C. A change in the size and orientation of the fiber cell nuclei (arrow) indicates that fiber cell differentiation has commenced and that cells have entered the meridional rows (MR). Scale bars in B and C are 250 µm, and in D is 50 µm.