Table 1 of Katoli, Mol Vis 2010; 16:1241-1252.

Table 1. Relative potencies and intrinsic activities of various NPs for their ability to stimulate cGMP production in human CEPI cells compared with other human ocular cell-types.



Natriuretic
peptide		 Potency (nM)
and intrinsic activity (Emax)
in p-CEPI
Cells		 Potency (nM)
and intrinsic activity (Emax)
in CEPI-17-CL4
Cells		 Potency
in h-CM
cells
(nM)#		 Potency
in h-TM16
cells
(nM)#		 Potency
in h-TM3
cells
(nM)#
CNP (1–53 amino acids)
EC50 (nM) 24 ± 5 nM 7 ± 2 nM** 19 nM 10 nM 64 nM
Max. effect (%) 100% 100%      
CNP fragment (32–53 amino acids)
EC50 (nM) 51 ± 8 nM 16 ± 2 nM** nd nd nd
Max. effect (%) 96% 89%      
BNP (32 amino acids)
EC50 (nM) >10,000 nM 203 ± 95 nM** 35 nM 75 nM 398 nM
Max. effect (%) 4.5% 43%      
ANP (1–28 amino acids)
EC50 (nM) >10,000 nM 97 ± 25 nM** 102 nM 102 nM 478 nM
Max. effect (%) 3.8% 13%      

Concentration-response curves were generated for each of the NPs shown above using 6 concentrations of each peptide in each experiment as shown in Figure 5B,C. Data shown above are mean±SEM from 3 to 4 experiments for human p-CEPI cells and from 4 to 9 experiments for CEPI-17-CL4 cells. #=data from [18] for comparison. h-CM=human ciliary muscle cells; h-TM=human trabecular meshwork cells; nd=not determined. **=p<0.01 relative to potency in p-CEPI cells by Student’s unpaired t-test.

Katoli, Mol Vis 2010; 16:1241-1252. http://www.molvis.org/molvis/v16/a137

©2010 Molecular Vision http://www.molvis.org/molvis/

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