Figure 5. A: Time-course of increase in cGMP induced by CNP fragment in CEPI-17-CL4 cells is shown. Cyclic GMP concentration was measured after the cells were treated with CNP fragment for the indicated period. Each symbol represents a single datum point determined in duplicate. B and C: Effects of NPs on cGMP production in human p-CEPI and CEPI-17-CL4 cells are shown. Cyclic GMP concentration was measured after the cells were treated with the indicated concentrations of various NPs for 15 min. Each symbol represents a single datum point determined in duplicate. Results were obtained in 4 independent studies for CEPI-17-CL4 cells (B) and 3 independent studies for human p-CEPI cells (C).