Figure 5. Hetero-oligomerization of βB1 dimers with βA3 dimers. In order to mix βB1 dimers with βA3 dimers, the concentration of βB1 was increased to favor βB1 dimer formation. A 5:1 molar ratio of βB1:βA3 was mixed and immediately subjected to SEC-MALS (bottom panel) or after 30 min at 37 °C (top panel). Molar masses are indicated by the closed squares and the UV absorbance at 280 nm by the grey tracing. Similar molar masses are obtained for the hetero-oligomer as in Figure 3.