Figure 1. Two-dimensional electrophoresis
(2-DE) profiles of total adult zebrafish lens protein. Separation was
performed on 11 cm IPG strips with pH gradients of 5–8 (
A), 3–10
nonlinear (
B) and 7–10 (
C). Gels were Coomassie stained
and spots were identified by MALDI-TOF MS. Mass standards are indicated
on the left in kiloDaltons. Spots labeled with a “?” contain two or
more crystallins from the indicated family. Accession numbers for each
crystallin are shown in
Table 1. The accession numbers
for four identified non-crystallins are as follows: actin (
AAO38846),
BTB (POZ) domain containing protein 2 (
NP_001038557),
proliferation associated nuclear panel 1 (Pane1;
NP_991153),
and galectin-related inter-fiber protein (
XP_684452).