Figure 4. MALDI-TOF MS spectrum of
biotin-labeled αB-crystallin tryptic peptides purified by avidin
affinity chromatography. Sulfo-SBED-derivatized βA3/A1
102–117
peptide was allowed to interact with αB-crystallin then was photolyzed
and digested with trypsin as described under Methods before
chromatography. Masses of the αB-crystallin tryptic peptides carrying
the biotin label are indicated in the figure. The sequences of these
peptides are shown in
Figure 5.
Figure 4 of Rao, Mol Vis 2008; 14:666-674. 