Figure 5. Amino acid sequence of
αB-crystallin showing the βA3/A1102–117 peptide interacting
sites (underlined). The chaperone region previously identified by us is
shaded in gray. Biotin-labeled αB-crystallin peptides were analyzed by
MALDI-TOF MS, and their sequences were assigned by nanospray QqTOF
MS/MS. The masses of the biotin-labeled αB-crystallin peptides are
shown adjacent to their sequences. *NH2-terminal peptide was
consistently observed in the MALDI-TOF MS without a sulfo-SBED label.
This peptide may be interacting with other labeled peptides and
co-eluting during the monomeric avidin purification step.