Figure 5 of Rao, Mol Vis 2008; 14:666-674.


Figure 5. Amino acid sequence of αB-crystallin showing the βA3/A1102–117 peptide interacting sites (underlined). The chaperone region previously identified by us is shaded in gray. Biotin-labeled αB-crystallin peptides were analyzed by MALDI-TOF MS, and their sequences were assigned by nanospray QqTOF MS/MS. The masses of the biotin-labeled αB-crystallin peptides are shown adjacent to their sequences. *NH2-terminal peptide was consistently observed in the MALDI-TOF MS without a sulfo-SBED label. This peptide may be interacting with other labeled peptides and co-eluting during the monomeric avidin purification step.