Appendix 5 of Sun, Mol Vis 2008; 14:2237-2262.

Appendix 5. Quantification of the effect of rcSF3 on the differentiation of E7 RPE in culture.

Select genes characterized in Figure 3 and Table 3 were examined to identify those that E14 rcSF3 upregulated or down-regulated more than 2X in cultures of E7 RPE. For comparison, the second column indicates how gene expression would increase or decrease during normal development [1]. The third and forth columns indicate the level of mRNA expression in different culture conditions relative to expression in vivo on E14. The last, fifth, column indicates the level of expression in rcSF3 cultures relative to SF3 cultures. For many genes, the E14-derived rcSF3 increased or decreased expression in E7 cultures in parallel with an increase or decrease that occurred during normal development. For many genes, the culture/native E14 RPE was closer to the ideal of 1.0 in the rcSF3 cultures. To access the data, click or select the words “Appendix 5.” This will initiate the download of a compressed (pdf) archive that contains the file.