Table 1 of Shiels, Mol Vis 2008; 14:2042-2055.


Table 1. PCR primers for mutation-profiling of EPHA2.

Primer Location Strand Sequence (5′-3′)
Ex1F Intron-1 Antisense gcgaccaagctgaaaccgcttatt
Ex1R 5′-upstream Sense ggcatgaatgaacaggagtcggtt
Ex2F Intron-2 Antisense cgtaccttcccacgcccatc
Ex2R Intron-1 Sense ccagcctgctgtgtgccttc
Ex3F1 Exon-3 Antisense ACGGCATGGTCCACACAGGT
Ex3R1 Intron-2 Sense ccaggcacctgcccacacta
Ex3F2 Intron-3 Antisense aaggaaactgatgtctgggaagga
Ex3R2 Exon-3 Sense CCAGAAGCGCCTGTTCACCA
Ex4F Exon-5 Antisense CAGACTCGGGCCAGCACTGT
Ex4R Intron-3 Sense ttcctgggtgcccggtacat
Ex5F Intron-5 Antisense gacactgtgcctttaaccacttgctc
Ex5R Exon-4 Sense GCTTCTTCCGGGCACCTCAG
Ex6F Intron-6 Antisense ctctgctgtgctgccttggg
Ex6R Intron-5 Sense tgcctgctcgtaggcagctt
Ex7F Intron-7 Antisense ccgccggtgaccgagaaag
Ex7R Intron-6 Sense ggcgtccttggaagaggcag
Ex8F Intron-8 Antisense gattcctgcctgcgtgtcca
Ex8R Intron-7 Sense tggagccttcccaagtgcaa
Ex9/10F Intron-10 Antisense accaccgctgcctcctca
Ex9/10R Intron-8 Sense tgggccgcattctgagcac
Ex10/11F Intron-11 Antisense cctctccacccagtgtgggc
Ex10/11R Intron-9 Sense cccacagcctggtccaagtc
Ex12F Exon-13 Antisense TGGTGGTGTAGGTGGCCTCG
Ex12R Intron-11 Sense tacctctgcccactcctccg
Ex13F Exon-14 Antisense CGTCGCTGGCAGAGGTGAAC
Ex13R Exon-12 Sense CCCTGGACAAGTTCCTTCGGg
Ex14F Intron-14 Antisense aactgtcctctgcccagccc
Ex14R Exon-13 Sense CGAGGCCACCTACACCACCA
Ex15F Intron-15 Antisense ctgggccatcgtgtccagtc
Ex15R Intron-14 Sense gggcagctctgaaggttggg
Ex16F Intron-16 Antisense tggcggagttctgcccttct
Ex16R Intron-15 Sense gactgggcttccctgttgcc
Ex17F1 Exon-17 Antisense agggaccgctttgggtctca
Ex17R1 Intron-16 Sense ctctccctctctccctcccg
Ex17F2 Exon-17 Antisense ccctgccacacacacacattc
Ex17R2 Exon-17 Sense agtggcctccctgctgtgc
Ex17F3 3′-downstream Antisense gctcccagggttaagtgacgtg
Ex17R3 Exon-17 Sense gcagactgtgaacttgactgggtga
T-alleleF Exon-17 Antisense GCCGGGCAGCCGCACCCA
Ex17SF Exon-17 Antisense ggaggccactctgtttcttcaagt