Molecular Vision: Shiels, Mol Vis 2008; 14:2042-2055. Table 1

Table 1. PCR primers for mutation-profiling of EPHA2.

Primer	Location	Strand	Sequence (5′-3′)
Ex1F	Intron-1	Antisense	gcgaccaagctgaaaccgcttatt
Ex1R	5′-upstream	Sense	ggcatgaatgaacaggagtcggtt
Ex2F	Intron-2	Antisense	cgtaccttcccacgcccatc
Ex2R	Intron-1	Sense	ccagcctgctgtgtgccttc
Ex3F1	Exon-3	Antisense	ACGGCATGGTCCACACAGGT
Ex3R1	Intron-2	Sense	ccaggcacctgcccacacta
Ex3F2	Intron-3	Antisense	aaggaaactgatgtctgggaagga
Ex3R2	Exon-3	Sense	CCAGAAGCGCCTGTTCACCA
Ex4F	Exon-5	Antisense	CAGACTCGGGCCAGCACTGT
Ex4R	Intron-3	Sense	ttcctgggtgcccggtacat
Ex5F	Intron-5	Antisense	gacactgtgcctttaaccacttgctc
Ex5R	Exon-4	Sense	GCTTCTTCCGGGCACCTCAG
Ex6F	Intron-6	Antisense	ctctgctgtgctgccttggg
Ex6R	Intron-5	Sense	tgcctgctcgtaggcagctt
Ex7F	Intron-7	Antisense	ccgccggtgaccgagaaag
Ex7R	Intron-6	Sense	ggcgtccttggaagaggcag
Ex8F	Intron-8	Antisense	gattcctgcctgcgtgtcca
Ex8R	Intron-7	Sense	tggagccttcccaagtgcaa
Ex9/10F	Intron-10	Antisense	accaccgctgcctcctca
Ex9/10R	Intron-8	Sense	tgggccgcattctgagcac
Ex10/11F	Intron-11	Antisense	cctctccacccagtgtgggc
Ex10/11R	Intron-9	Sense	cccacagcctggtccaagtc
Ex12F	Exon-13	Antisense	TGGTGGTGTAGGTGGCCTCG
Ex12R	Intron-11	Sense	tacctctgcccactcctccg
Ex13F	Exon-14	Antisense	CGTCGCTGGCAGAGGTGAAC
Ex13R	Exon-12	Sense	CCCTGGACAAGTTCCTTCGGg
Ex14F	Intron-14	Antisense	aactgtcctctgcccagccc
Ex14R	Exon-13	Sense	CGAGGCCACCTACACCACCA
Ex15F	Intron-15	Antisense	ctgggccatcgtgtccagtc
Ex15R	Intron-14	Sense	gggcagctctgaaggttggg
Ex16F	Intron-16	Antisense	tggcggagttctgcccttct
Ex16R	Intron-15	Sense	gactgggcttccctgttgcc
Ex17F1	Exon-17	Antisense	agggaccgctttgggtctca
Ex17R1	Intron-16	Sense	ctctccctctctccctcccg
Ex17F2	Exon-17	Antisense	ccctgccacacacacacattc
Ex17R2	Exon-17	Sense	agtggcctccctgctgtgc
Ex17F3	3′-downstream	Antisense	gctcccagggttaagtgacgtg
Ex17R3	Exon-17	Sense	gcagactgtgaacttgactgggtga
T-alleleF	Exon-17	Antisense	GCCGGGCAGCCGCACCCA
Ex17SF	Exon-17	Antisense	ggaggccactctgtttcttcaagt

Gene specific primers used for amplification and sequencing of exons 1–17 and flanking intron regions of EPHA2 on chromosome 1p. Coding and noncoding sequence are shown in uppercase and lowercase, respectively. Antisense primers were 5′-tailed with the M13-forward universal sequence (5′-tgtaaaacgacggccagt), and sense primers were 5′-tailed with the M13-reverse universal sequence (5′-caggaaacagctatgacc). The allele-specific primer (T-alleleF) was used to detect the heterozygous c.2842G>T transversion in exon-17 as shown in Figure 3.