Figure 2. Assessment by qPCR of expression changes in genes identified by microarray analysis as hyperoxia-regulated. As noted for Table 5, these data validate trends in the Genechip data. A: Validation of human retinal disease genes showing upregulation of C3, downregulation of DMD and no change to Prpf3. B: Control genes (opsins) which showed no change in expression. C-E: Additional sets of genes assessed by qPCR. C shows a group of three genes upregulated at 14 day. D shows a group upregulated at all time points; E shows contrasts a gene (Oxr1) upregulated at 3 day, with GFAP, which is maximally upregulated at 14 day. For each gene, the fold change in expression was determined using 0 day expression as control and normalizing the data to the housekeeping gene GAPDH. Fold changes below the dashed line indicate expression decrease while above the line indicate expression increase. Error bars show standard error of the mean.