Molecular Vision: Natoli, Mol Vis 2008; 14:1983-1994. Table 5

Table 5. Comparison of Genechip and qPCR data.

Gene	qPCR			GeneChip
Gene	Day 3	Day 7	Day 14	Day 3	Day 7	Day 14
BCL-3	1.21	1.03	3.66	NC	NC	4.1
C3	−1.11	1	2.8	NC	−6.21	10.42
Edn2	1.46	1.48	4.71	2.93	NC	13.15
Gadd45b	1.37	1.77	3.35	NC	NC	4.54
GFAP	−1.33	2.14	7.2	NC	2.31	16.64
HIF1a	1.5	1.4	1.1	3.48	NC	−3.37
Lin7b	1.72	1.45	1.96	NC	NC	4.39
L/M-opsin	1.4	1.2	1.1	NC	NC	NC
Oxr1	1.83	1.08	−1.25	3.3	NC	−2.6
Rhodopsin	−1.11	1	−1.11	NC	NC	NC
Siva	1.47	1.45	1.42	NC	NC	2.3
S-opsin	1	1	1	NC	NC	NC
DMD	−1.25	−1.23	−2	NC	NC	−5.38, −2.3, −4.13
Prpf3	1.1	1	1	NC	NC	−5.2
GAPDH*	1	1	1	NC	NC	NC

Generally, genes showed the same trend in regulation in the qPCR and microarray experiments, but not necessarily by the same fold change. No change (NC) refers to a gene whose expression was below twofold for the microarray experiment and therefore the results were not statistically significant. The asterisk indicates a housekeeping gene. Validation, using qPCR, of genes linked to human retinal diseases were found to have differential expression at day 14 exposure to hyperoxia. Multiple entries indicate multiple probe identification (ID)’s from the GeneChip® analysis. Note that Prpf3 did appear to change when tested using qPCR.