Table 2 of Bowne, Mol Vis 2008; 14:922-927.

Table 2. Probes used in multiplex ligation probe amplification

Probe set Probe location* 5′ half probe (5′-3′) Phosphorylated 3′ half probe (5′-3′)
Amp1 Front 32542621–32542674 GGGTTCCCTAAGGGTTGGA TGGCGGGTACACCCAGC AGCCCTTAC pGTAAGAAGCGGAAGATCGTATCCT CCAGTCTAGATTGGATCTTGCTGG CAC
Amp 1 Back 32542362–32542421 GGGTTCCCTAAGGGTTGGA GGCAGCAGTCCGCGGGA GCTGGCGGGAG pCTGCGGGCCTTACAGTAACCTCCC AGGCGGTGTCTAGATTGGATCTTGCTGGCAC
Amp 2 Front 32540968–32541035 GGGTTCCCTAAGGGTTGGA CTGGGGGGTCTCGCTCTC TGCCCTGCTTCCGAG pCTGCCATTGGTGATGAGCCCTTTG CGTCACATCTAGATTGGATCTTGC TGGCAC
Amp 2 Back 32540731–32540800 GGGTTCCCTAAGGGTTGGA CAGCCAGGCCTGCGCCG GCATCCTCCGAG pGTGAGTGAGATTCGGAACAAT GGG ACGCGGGGGTCGGAAGGTCTAGATTGGATCTTGCTGGCAC
Amp 3A Front 32534380–32534475 GGGTTCCCTAAGGGTTGGA CAGTGCCCCTTTATAAA ATAAAACAAAAGTAATGG GTCACTTAAGTATTTTCAC pCAAAATAAGTTTCAAGGTCTTTAT TTGCATTTTTGTTGAGACTCTAGATTGGATCTTGCTGGCAC
Amp 3A Back 32532520–32532601 GGGTTCCCTAAGGGTTGGA GAGACAAAAAGAGATCA AGAACTAGAGATAGCAG TTGGTC pCAGAAGAAGCCAAACTCTGTCTCT AAGTAGTGAAAGCACAAGTCTAGATTGGATCTTGCTGGCAC
Amp 3B Front 32533017–32533090 GGGTTCCCTAAGGGTTGGA GTGTCATTGTTGGGTTTGTT AAACCACTAGCTGAGAG pGACCCCAGAACTTGTTGAACTGTC CTCTGATTCTGAGTCTAGATTGGATCTTGCTGGCAC
Amp 3B Back 32531257–32531342 GGGTTCCCTAAGGGTTGGA GGAAAAAGGAAGAATGT CGTCTACTGCAGTCTATT TAAAGAT pGACATTTGGTGAAAACTCTCTTCC TCCTTACAATATTTTAAATGTCTAGATTGGATCTTGCTGGCAC

Half probes were designed to anneal to the equivalent genomic sequence as the sequencing amplification primers described in Table 1. Each probe sequence contains a universal half probe sequence which is underlined. The asterisk indicates the position on chromosome 9 based on the University of California Santa Cruz human genome assembly of March 2006 (hg18).

Bowne, Mol Vis 2008; 14:922-927. http://www.molvis.org/molvis/v14/a110

©2008 Molecular Vision http://www.molvis.org/molvis/

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