Table 1 of Bowne, Mol Vis 2008; 14:922-927.

Table 1. Primers used in polymerase chain reaction amplification and sequencing

Exon Amplification primers (5’-3’) Annealing temperature Sequencing primers (5’-3’)
1 ACGTAAGAAGCGGAAGATCG 63 °C Same as amplification

GCCTGGGAGGTTACTGTAAGG

2 GTGGGTCTC GCT CTC TGC 63 °C Same as amplification

CCCATTGTTCCGAATCTCAC

3A TCAAGGTCTTTATTTGCATTTTTG 52 °C TCAAGGTCTTTATTTGCATTTTTG

GCTTCTTCTGGACCAACTGC
AGAACAACAACTCCACCG



GCCTTCACAGATTAGTCCC



GAGAAACGATCTACATCATTGTC



AGTTGGCCTCCTTACTGCAA



GACCACTCCTGTACACAGCGAAAAC



TTCTGGGGTCCTCTCAGCTA



GGCTTCTTCTGGACCAACTGC
3B TAGCTGAGAGGACCCCAGAA 58 °C AGTTGGTCCAGAAGAAGCCA

GGAGGAAGAGAGTTTTCACCAA
TACAAAACACGGCATTTGGA



AAGACCCGGAGCCTAAGTGT



GATGAAGATTTTTGGTAATGACTG

The above PCR and sequencing primers were used to identify TOPORS mutations in our cohort of adRP patients. For exons 1 and 2, the same primers were used for amplification and sequencing reactions. For exons 3A and 3B, several nested sequencing primers were used to span each amplified PCR product.

Bowne, Mol Vis 2008; 14:922-927. http://www.molvis.org/molvis/v14/a110

©2008 Molecular Vision http://www.molvis.org/molvis/

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