Figure 2. Immunocytochemical analysis of caveolin-2 in the human retina

Samples were taken as described in Figure 1. Alexa Fluor 488 was used to detect caveolin-2 (green). The cytoskeleton and the nuclei were treated with Alexa fluor 594 labeled phalloidin (red) and DAPI (blue), respectively. Merged images are shown. Caveolin-2 was found in the outer nuclear layer (ONL) and the inner limiting membrane (ILM), including the intervening layers. A, D, and F reveal: very low, low, and moderate densities (M). B, E: At the peripheral part (P) immunoreactivities (IRs) were observed only in the outer nuclear layer (ONL), outer plexiform layer (OPL), inner nuclear layer (INL), and ganglion cell (GC; very low, low). C: No signals were observed at the epithelium of the ciliary body (CB). Note, that pigment granules show autofluorescence. Scale bar represents 10 μm. The following abbreviations used in this figure: pigment epithelium (PE), outer segments (OS), outer limiting membrane (OLM), inner nuclear layer (IPL), optic nerve fibers (ONF).