Figure 7. Gene expression in two DB3 cells

Gene-specific PCR was performed using cDNA libraries created using TPEA RT-PCR on RNA extracted from two representative cells, as shown in Figure 4. A, F: Ethidium bromide-stained 2% agarose gels for two cells harvested based on immuno-label for calbindin demonstrate expression of the calbindin gene, but not GAD-65 or GAD-67, which would be expressed in horizontal cells. We confirmed this pattern for 26 of 34 harvested DB3 cells and used gene-specific PCR from the same cDNA libraries to test expression of AMPA (B, G), kainate (C, H), and mGluR subunits (D, I). We also tested the expression of the five EAAT subunits (E, J). Arrowhead in each gel indicates 300 bp.