Figure 1. Positive and negative controls using cDNA from fixed retinal slices

A: Ethidium bromide-stained 2% agarose gel shows gene-specific PCR from a cDNA library created using 3'-end amplification (TPEA) RT-PCR for RNA extracted from a fixed slice from Macaca nemestrina retina. Results demonstrate positive expression of each GluR, mGluR, and transporter subunit tested using the primers listed in Table 1 B: The same PCR reactions are shown, but for a fixed slice from a second Macaca nemestrina retina. The results are similar to the first retina. C: The same reactions are shown, but with reverse transcriptase omitted as a negative control. A single PCR product was detected in each reaction, so gels are truncated. Arrowheads indicate 300 bp.