Table 1 of
Hanna, Mol Vis 2007;
13:2194-2208.Table 1 of
Hanna, Mol Vis 2007;
13:2194-2208.
Table 1. Primers for gene-specific polymerase chain reaction in single bipolar neurons
The primers were used to detect genes from mRNA extracted from single bipolar cells previously labeled using immunocytochemistry in fixed retinal slices. "Accession number" is the GenBank accession number for the corresponding human gene. "Location" is the position of initial nucleotide for forward and reverse primer pairs. "Human sequence" represents the sequence for design of forward and reverse primers. "Size" is the number of base pairs in expected PCR product.
Accession Size
Gene number Location Human sequence (5'-3') (bps)
---------------- --------- -------- ------------------------ -----
GluR1 NM_000827 2071F TGGTGTCTCCCATTGAGAGTGC 188
2258R AATCATCCCCTCCTCTGTGGTC
GluR2 NM_000826 1559F TTTTTGCCTACATTGGGGTCAG 184
1742R TAGTCTCTGGTTTTCCTTGGGTGC
GluR3 NM_000828 2072F CAGACTGAAATTGCATATGGGACC 191
2262R TCCAGCAGGAAGGCAAACTTTC
GluR4 NM_000829 2068F TGAAAAGATGTGGACCTACATGCG 186
2253R GAATCCAGATTTCCTCCCACTTTC
GluR5 NM_000830 2428F TCTGGCTGCCTTCTTGACAGTAG 204
2631R TCACTGTTTCTTACCAGGGCGG
GluR6 NM_021956 1977F GACAGTGGAACGCATGGAATCC 206
2182R CTCGCTGGATTCCTTCTTCATTAC
GluR7 NM_000831 2153F CTGATGACCTGGCCAAGCAAAC 232
2384R TTCCTCTGCGTGACGTACTCGATG
KA1 NM_014619 2218F GGGAATCGCCAGGGTGTTGAATCC 216
2433R TTCAGGATCTCCAGGCGGTTGTTC
KA2 NM_002088 2194F CTCAACTGCAACCTCACCCAGATC 205
2398R CCAAACCTTTAGCTCGATGGTCC
mGluR1 NM_000838 2900F AATGGCAAGTCTGTGTCATGGTC 186
3085R GCTGGTATCTGAAAAGGTCAGGC
mGluR2 NM_000839 2043F CCATCTGCCTGGCACTTATCTC 199
2241R AAGGCATAAAGCGTGCAGAGC
mGluR3 NM_000840 2047F ATCAAGCACAACAACACACCCTTG 186
2232R CAGCAGGGCTGAGTAACAGATAGC
mGluR4 NM_000841 2683F ACATGCCCAAAGTCTACATCATCC 199
2881R TAAGTCTGTTTGGTGGCCAGCG
mGluR5 NM_000842 2019F TGGCATCTGCCTGGGCTACTTATG 205
2223R ACAGGCACTCATGCCTCTGGGC
mGluR6 NM_000843 2302F AGGTGGTGGGGATGATAGCATG 190
2491R TTGATGGCGTACACTGTGCACG
mGluR7 NM_000844 2005F CTGGGCTGTGATTCCTGTCTTC 225
2229R AAGCCCAAGAAAACTCGCCG
mGluR8 NM_000845 2001F CTCCTTCCGACGGGTCTTCCTA 181
2181R AAGGAGCTGGACGGAGATGA
EAAT1
(GLAST) NM_004172 819F TAAACAATGTGTCTGAGGCCATGG 207
1025R CTACCAGTCTCATGATGGCTTCG
EAAT2
(GLT-1) NM_004171 1256F TTCATTGCTTTTGGCATCGC 191
1446R TGCCTAGCAACCACTTCTAAGTCC
EAAT3
(EAAC-1) NM_004170 808F ATTCAGATGGCATAAACGTCCTGG 188
995R CCGAGCAATCAGGAACAAAATACC
EAAT4 NM_005071 706F GAACCAGCTTCCTGGAAAATGTC 202
907R TTGAGGCTGTCGAAGAAGTCCCTG
EAAT5 NM_006671 877F TCAGCTTCTGCCAGTGCCTCAATG 208
1084R TAGAGCAGGGGCAGGATAAAGAGC
PKCα XM_034737 1045F TCCTCATGGTGTTGGGAAAGG 212
1256R ATCCACTGTCTGGAAGCAGGAGTG
Gao NM_020988 522F CATCCTCCGAACCAGGGTCAAAAC 192
713R TCTTCGTGGAGCACCTGGTCATAG
Calbindin NM_004929 667F TGGAATTAACTGAGATGGCCAGG 175
841R TCGCACAGATCCTTCAGTAAAGC
GAD-65 XM_038553 949F TGCTCTTCCCAGGCTCATTG 194
1142R TCACGAGGAAAGGAACAAACCC
GAD-67 NM_000817 1585F TGGCACGACTGTTTATGGAGC 163
1747R GACTGAGTTGGCCCTTTCTATGC
β-actin NM_001101 411F 5'-AGGCCAACCGCGAGAAGATG 123
533R 5'-CCATCACGATGCCAGTGGTA
Glutamine Synth. NM_002065 1239F 5'-GGGAACTGGAATGGTGCAGG 110
1348R 5'-TGCCGCTTGCTTAGTTTCTC
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