Figure 5. Copy number of CD4 and CD8 transcripts in corneas of naive mice

Corneas and spleens from individual naive BALB/c and C57BL/6 mouse were isolated as described in the legend to Figure 3. TaqMan RT-PCR was performed on total RNA and GAPDH expression was used to estimate the relative expression of CD4 and CD8 mRNA in each individual mouse corneas. As positive control BALB/c and C57BL/6 mice were ocularly infected with 2x10⁵ PFU/eye of highly neurovirulent HSV-1 strain McKrae. Infected mice were euthanized on day 5 post-infection, the corneas of each individual mouse were isolated, and TaqMan RT-PCR was performed on total RNA as above. Each point represents mean±SEM from eight and five mice for naive and infected mice, respectively. Panels: A CD4 copy number in cornea; B CD8 copy number in cornea; and C CD4 and CD8 copy number in spleen.