Figure 3 of Mott, Mol Vis 2007; 13:1802-1812.


Figure 3. Detection of CD4+ and CD8+ T cells in the intact corneas of naive mice by confocal microscopy

Eight-week-old, female, naive BALB/c, C57BL/6, and SCID mice were euthanized, and their eyes were immersed in 2% paraformaldehyde as described Methods. Cross-sectional projections of BALB/c (A and B), C57BL/6 (C and D), and SCID (E and F) mouse corneas stained with Alexa Fluor 488-conjugated anti-CD4 (A, C, and E) or FITC-conjugated anti-CD8 (B, D and F) monoclonal antibodies are shown. Positive staining appears green. Inserts (A, C and D) show positively stained cells as they appeared in individual optical planes (insert zoomed 2x from projection magnification). Tissue was counter-stained with DAPI to indicate cell nuclei (pseudocolored in red).

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Mott, Mol Vis 2007; 13:1802-1812 <http://www.molvis.org/molvis/v13/a201/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535