Table 1 of
Lopez-Garrido, Mol Vis 2006;
12:748-755.Table 1 of
Lopez-Garrido, Mol Vis 2006;
12:748-755.
Table 1.
Oligonucleotide primers used for amplification of the CYP1B1 gene
Annealing
Primer sequence (5'->3') temperature/
Primer ---------------------------------------------------- time Extension
set Region Forward Reverse (°C/s) time (s)
------ -------- --------------------------- ---------------------- ------------ ---------
1 Exon II TCTCCAGAGAGTCAGCTCCG GGGTCGTCGTGGCTGTAG 57/ 5 45
2 Exon II ATGGCTTTCGGCCACTACT CGATCTTGGTTTTGAGGGGTG 57/ 5 45
3 Exon III AATAAGAATTTTGCTCACTTGCTTTTC AGCAGCACAAAAGAGGAACTGG 61/20 40
4 Promoter ATGACTGGAGCCGACTTTCC GCAAAGTCGAGGTTTCCTCACA 58/20 50
5 Exon I CCTCCTTCTACCCAGTCCTTAAAA TAAAGTACCTACCACGCCACCC 57/20 20
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