Table 1 of
Steely, Mol Vis 2006;
12:372-383.Table 1 of
Steely, Mol Vis 2006;
12:372-383.
Table 1. A listing of NCBI accession numbers, names, and analytical information for GTM3 proteins identified from 2-D gels
NCBI accession numbers, names, and analytical information for primary proteins identified in the spots labeled in Figure 1. The molecular weights (MW) are monoisotopic. The indicated NCBI MW and pI values are listed from the NCBI database. The estimated MW and pI values were obtained by interpolation as experimental values based on the position of each spot on the gel, which were estimated using an interpolation algorithm in the Gellab II+ software. The NCBI data for spot numbers marked in red were used to calibrate MW and pI axes. The "Number ions" column denotes the number of unique peptides identified arising as tryptic fragments of the indicated protein with χcorr>1.8, 2.5, and 3.5 for single, double, and triple charged ions, respectively. Sample pooling was used for entries marked with an asterisk. The "% amino acid" column denotes percentage of the total protein amino acids that were contained in the peptides counted in the "Number ions" column, specifically, as the percentage of total possible amino acids. ETEF: eukaryotic translation elongation factor, HNRP: heterogeneous nuclear ribonucleoprotein, HSP: heat shock protein, VCP: valosin-containing protein, VDAC: voltage-dependent anion-selective channel.
NCBI %
Spot accession NCBI Estimated NCBI Estimated Number amino
number number Protein name MW MW pI pI ions acid
------ ---------- -------------------------- ----- --------- ---- --------- ------ -----
1 NP_005057* Splicing factor 76103 75850 9.45 9.94 6 10.6
proline/glutamine rich
2 NP_005372* Nucleolin 76299 75245 4.59 4.68 7 13.2
3 NP_003676* KH-type splicing 73117 72662 6.84 6.98 16 33.2
regulatory protein
4 NP_003866 Guanine monophosphate 76668 71180 6.42 6.85 22 46.6
synthetase
5 NP_004125 Heat shock 70 kDa protein 73636 71099 5.87 5.5 20 43
9B precursor
6 NP_006588 Heat shock 70 kDa protein 70855 70855 5.37 5.23 26 52.3
8 isoform 1
7 NP_003893 Far upstream 67520 71417 7.18 7.32 10 21.6
element-binding protein
8 NP_116126* Lamin B2 67649 68261 5.29 5.37 11 29.2
9 AAH07562* VCP protein 64750 64804 5.09 5.14 16 46.8
10 NP_006810 Hsp70/Hsp90-organizing 62600 64951 6.4 6.4 17 32.6
protein
11 NP_112553* HNRP K 50997 64512 5.19 5.43 14 44.2
12 NP_002147* Heat shock 60 kDa protein 61017 62081 5.7 5.22 19 50.4
1 (chaperonin)
13 NP_000909 Prolyl 4-hydroxylase, beta 57081 62222 4.76 4.61 19 49.2
subunit
14 NP_036205* Chaperonin containing 59634 62222 5.45 5.48 27 59
TCP1, subunit 5 (epsilon)
15 NP_001753 Chaperonin containing 57989 62081 6.24 6.38 6 21.1
TCP1, subunit 6A (zeta 1)
16 NP_006073 Tubulin, alpha 50121 58143 4.94 4.71 14 41.2
17 NP_003371 Vimentin 53654 58143 5.06 4.71 9 27
18 AAM47026 Desmin, mutant 53217 56844 5.25 5.28 16 45
19 NP_006614 3-Phosphoglycerate 56615 57231 6.26 6.39 18 47.7
dehydrogenase
20 P12268 Inosine-5'-monophosphate 55771 57231 6.44 6.78 13 43.8
dehydrogenase 2
21 NP_002645 Pyruvate kinase 3 isoform1 57901 57360 7.96 7.43 13 36.2
22 NP_005601 Retinoblastoma binding 47627 54453 4.74 4.59 10 49.7
protein 4
23 P30101 Protein 56762 56716 5.98 6.23 25 63.6
disulfide-isomerase ER60
precursor
24 NP_055317* Nuclear matrix protein 55147 53719 6.14 6.36 3 14.3
NMP200
25 AAH20946 Tubulin, beta 49641 48849 4.75 4.67 18 66.4
26 NP_006422 Chaperonin containing 57453 51575 6.01 6.33 13 43.9
TCP1, subunit 2 (beta)
27 NP_001148 Annexin A11 54356 48705 7.53 7.22 9 21.8
28 NP_003698* TATA binding protein 50197 48228 6.02 6.37 13 43.2
interacting protein 49 kDa
29 NP_003356* Ubiquinol-cytochrome c 52613 46504 5.94 5.56 4 12.7
reductase core protein I
30 NP_004334 Calreticulin precursor 48113 45868 4.29 4.22 7 31.7
31 AAB97725 Calumenin 37051 45823 4.47 4.47 4 20.6
32 P52597 HNRP F 45644 41711 5.38 5.24 13 55.4
33 NP_001419 Enolase 1 47140 44800 7.01 7.01 18 61.3
34 NP_001092 Actin, beta (gamma) 41711 41711 5.29 5.24 15 59.2
35 NP_002286* Laminin receptor 1 32834 40507 4.79 4.61 10 47.1
36 NP_003312 Tu translation elongation 49844 41711 7.26 6.85 12 40
factor
37 AAF29073 HSPC108 37123 39339 5.83 5.35 11 52.9
38 NP_031401* TAR DNA-binding protein-43 44712 38297 5.86 6.26 6 32.1
39 NP_000282 Phosphoglycerate kinase 1 44587 39052 8.3 8.27 17 52.3
40 NP_000010 Acetyl-CoA 45172 37101 8.98 8.03 6 23.2
acetyltransferase 1
precursor
41 NP_005882 Acetyl-CoA 41270 35768 6.27 6.4 9 51.6
acetyltransferase 2
42 AAH03394 HNRP C 32319 35507 4.94 4.67 9 25.3
43 AAQ24860 Nucleophosmin [Homo 32584 33651 4.67 4.64 7 31
sapiens]
44 NP_001830 Calponin 3 36392 33373 5.69 6.25 9 41
45 AAB88178 alpha enolase 32287 34231 6.53 6.37 8 39.9
46 NP_006187 Poly(rC) binding protein 1 37503 33569 6.66 6.97 13 68.8
47 NP_000025 Aldolase A 39396 33316 8.3 8.31 19 73.4
48 NP_003748 ETEF 3, sub. 2 beta, 36 36480 32940 5.38 5.49 13 63.7
kDa
49 NP_001951 ETEF 1 delta isoform 2 31104 32733 4.9 4.67 6 31.7
50 NP_036269* Dimethylarginine 31103 32650 5.53 5.56 7 34
dimethylaminohydrolase 1
51 NP_919223* HNRP A3 39571 30346 9.1 7.5 5 17.7
52 O14979* HNRP homolog JKTBP 33569 32200 6.85 6.85 8 29.9
53 NP_006126* F-actin capping protein 32903 31240 5.45 5.42 11 66.1
alpha-1 subunit
54 NP_002583 Proliferating cell nuclear 28751 30965 4.57 4.56 14 73.2
antigen
55 NP_000691 Annexin I 38691 30693 6.57 6.39 11 41.6
56 NP_004359* Calponin 2 33676 30770 6.94 6.97 3 14.2
57 NP_002037 Glyceraldehyde-3-phosphate 36032 30385 8.57 8.65 8 41.7
dehydrogenase
58 NP_002128* HNRP A2/B1 35985 30384 8.67 8.65 7 32
59 NP_004030* Annexin II 38581 30346 7.57 7.5 7 24.8
60 NP_066952* Inorganic pyrophosphatase 32640 30499 5.54 5.59 13 59.2
61 NP_112533* HNRP A2/B1 37408 30423 8.97 10.03 10 44
62 AAG29873* Valosin-containing protein 34371 30385 6.08 5.68 4 27
63 NP_002127 HNRP A1 34176 29890 9.27 10.14 10 41.9
64 NP_036457* Microtubule-associated 29981 29516 5.02 4.71 4 29.8
protein, RP/EB family
65 AAC25675* Nuclear chloride ion 26908 28708 5.02 5.18 6 32.8
channel protein
66 AAH12883 VDAC2 protein 30394 37101 6.81 8.03 7 42
67 NP_004921* F-Actin capping protein 30610 27399 5.69 5.74 5 22.1
beta subunit
68 NP_006089* Guanine nucleotide binding 35056 25852 7.6 7.59 13 61.2
protein
69 NP_036220* 6-Phosphogluconolactonase 27531 27055 5.7 5.98 3 22.9
70 NP_039268* Dimethylarginine 29627 26885 5.66 5.74 8 49.5
dimethylaminohydrolase 2
71 NP_055227 Calcyclin binding protein 26195 26514 8.28 8.22 6 42.1
72 NP_006808* Endoplasmic reticulum 28976 26653 6.77 6.32 6 36.8
protein 29 precursor
73 NP_002625 Prohibitin 29787 26116 5.57 5.48 15 71.7
74 NP_002620 Phosphoglycerate mutase A, 28787 25949 6.67 6.95 6 37.4
nonmuscle
75 NP_001531 Heat shock 27 kDa protein 22770 25543 5.98 6.28 12 74.2
1
76 NP_004896 Peroxiredoxin 6 25020 25343 6 6.36 8 39.3
77 AAH17917 Triosephosphate isomerase1 26626 24173 6.45 6.31 14 78.3
78 NP_005380* Protein-L-isoaspartate 24622 24097 6.7 7.03 5 40.5
O-methyltransferase
79 NP_004484 Hydroxyacetyl-CoA 26907 23833 7.65 7.26 10 53.3
dehydrogenase, type II
80 NP_054817 Peroxiredoxin 3 isoform b 25823 23386 7.04 6.3 8 55.9
81 NP_002565 Peroxiredoxin 1 22097 22097 8.27 8.27 15 80.4
82 NP_009193 DJ-1 protein 19880 22072 6.33 6.32 9 60.9
83 NP_005800 Peroxiredoxin 2 isoform a 21879 21184 5.66 5.53 12 67.2
84 NP_000933* Peptidylprolyl isomerase B 23729 18708 9.42 10.2 3 14.8
(cyclophilin B)
85 NP_005498 Cofilin 1 (non-muscle) 18492 17175 8.22 8.17 9 61.5
86 NP_005554 Stathmin 1 17293 17293 5.76 5.91 7 41.6
87 NP_066953 Peptidylprolyl isomerase A 18002 16335 7.68 7.46 6 37.6
(cyclophilin A)
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