Figure 3. Double-labeling of late RPCs after sorbitol treatment with different cell specific markers

Panels A, D, G, J, M, and P (600x) show fluorescent images of Ki67, nestin, CRALBP vimentin, and PKCα immunreactivity while (B, E, H, K, N, Q, 600x) show that the same RPCs express GFAP or vimentin. (C, F, I, L, O, R, 600x) represent merged images of (B, E, H, K, N, Q) and (C, F, I, L, O, R). After 7 days of treatment 100% of the cells that expressed CRALBP also expressed GFAP and vimentin (G-I, J-L). However, not all the GFAP-positive cells were CRALBP-positive (G-I). In contrast, all the GFAP-positive cells were vimentin-positive (M-O). In addition, nestin and GFAP co-expression was observed, although the overlap was not 100% (Figure 3D-F), while Ki67 or PKCα were never seen to co-localize with GFAP (A-C, P-R).