Figure 4. The inhibitory effect of p44 mutants on phosphorylated and unphosphorylated WT rhodopsin

A: p44-mediated rhodopsin deactivation. Assays were performed as described in Figure 2 legend. p44 was added to a final concentration of 2 μM (phosphorylated rhodopsin) and 5 μM (unphosphorylated rhodopsin). Error bars represent SD (n=3-4). B: Direct binding of p44 mutants to different phosphorylation and photoactivation states of rhodopsin. Assays were performed as described in Figure 2 legend. All binding was normalized to binding of WT-Arr to R*P.