Figure 1.

RT-PCR analysis of SLRPs. SLRPS were amplified from cDNA Pool A (A), Pool B (B), and Pool C (C) by reverse transcription PCR, using primers from Table 1. Pool A RNA was extracted from of a 35-year-old woman, pool B RNA was extracted from three eyes of donors aged 40-60 years of undetermined gender, and pool C RNA was extracted from a 58-year-old male. cDNA was electrophoresed on 1% agarose gels, digitized, and analyzed in NIH Image, version 1.62. In the image, PRELP=proline/arginine end leucine-rich proteoglycan; Osteo=osteoglycin; Lum=lumican; G3PDH=glyceraldehyde 3-phosphate dehydrogenase, Dec=decorin; chon=chondroadherin.