Table 1 of Johnson, Mol Vis 2006; 12:1057-1066.


Table 1.

Primers specific for decorin, fibromodulin, chondroadherin, PRELP, osteoglycin, and lumican were designed to amplify cDNA of approximately 300 bp within the coding region of each gene. All primer sets were designed using MacVector 4.1 and purchased through Sigma-Genosys. In the "Primer sequence" column, "F" refers to the forward strand and "R" refers to the reverse strand.

                                                       Product    Annealing
                                                        size     temperature
     SLRP                  Primer sequence              (bp)        (°C)
--------------   -----------------------------------   -------   -----------
Chondroadherin   F: 5'-AGAAGATCCCCAAGGTGTCAGAG-3'        268         54
                 R: 5'-TTGTGGTCCAGGTAGAGGTAGGTC-3'

Decorin          F: 5'-ACATCCTCAGCTTTGAGGGCTCC-3'        374         56
                 R: 5'-CCAAATCAGAACACTGGACCACTC-3'

G3PDH            Primers from Clontech (human G3PDH)     983         68

Lumican          F: 5'-AGTGTACCAATGGTGCCTCCTG-3'         547         56
                 R: 5'-CCAGTTCGTTGTGAGATAAACGCAG-3'

Osteoglycin      F: 5'-GCTGACTGCCAAAGATTTTGCAG-3'        373         53
                 R: 5'-AGCCTTGCAGAATGTGTCATCTG-3'

PRELP            F: 5'-TACCTGCGGCTGGATGGAAACTAC-3'       305         60
                 R: 5'-TGGTTGTCTTTCAACTGGGTGGAAC-3'

Johnson, Mol Vis 2006; 12:1057-1066 <http://www.molvis.org/molvis/v12/a119/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535